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#bacterial stress

2 public questions tagged with this topic.

The heat shock response in bacteria involves:

Sudden increase in ambient temperature from 30 to 42 degrees Celsius and beyond causes widespread protein unfolding, misfolding and aggregation, exposing normally buried hydrophobic patches that drive non-native interactions and toxic oligomerization threatening proteome integrity. Bacterial heat shock response is orchestrated by alternative sigma factor sigma32 encoded by rpoH gene, whose expression is regulated at multiple levels including translation efficiency enhanced at high temperature via melting of inhibitory mRNA secondary structure and protein stability controlled by DnaK-DnaJ chaperone sequestration and FtsH proteolysis. When active, sigma32 directs core RNA polymerase to promoters of heat shock genes, massively upregulating molecular chaperones GroEL-GroES that provide ATP-dependent Anfinsen cage isolation for folding of 10 percent of proteome, DnaK-DnaJ-GrpE Hsp70 system that binds extended hydrophobic segments preventing aggregation and assisting refolding, ATP-dependent Clp proteases such as ClpP-ClpX and ClpB disaggregase that degrade irreparably damaged proteins, and Lon protease. This integrated network restores proteostasis, prevents inclusion body formation and permits survival. Increased ribosome synthesis would enhance growth not protection, decreased membrane fluidity would exacerbate stress, decreased ATP production reflects energy failure, so chaperone upregulation defines adaptive heat response signature conserved from bacteria to humans.

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 7: Heat Shock Response and Chaperone Upregulation.

The primary function of glutathione in bacterial stress response is:

Glutathione is a low-molecular-weight tripeptide gamma-glutamyl-cysteinyl-glycine present at millimolar concentrations up to 10 mM in many Gram-negative bacteria such as Escherichia coli and few Gram-positives that synthesize it via GshA and GshB. Reduced form GSH serves as major thiol buffer and electron donor protecting against reactive oxygen species generated continuously by endogenous respiratory electron transport and by host immune oxidative burst involving NADPH oxidase-dependent production of superoxide during phagocytosis. It directly scavenges superoxide anion, hydroxyl radical and hydrogen and organic peroxides via glutathione peroxidases, forming oxidized glutathione disulfide GSSG that is rapidly recycled by NADPH-dependent glutathione reductase Gor maintaining high GSH to GSSG ratio of greater than 100 to 1. GSH also forms mixed disulfides with redox-sensitive cysteine residues in proteins via reversible S-glutathionylation, temporarily shielding them from irreversible overoxidation to sulfinic or sulfonic acids that require repair. It acts as cofactor for glutathione peroxidases, glyoxalases that detoxify methylglyoxal, and for detoxification of electrophiles and xenobiotics via glutathione S-transferases. Depletion via mutation in gshA renders cells hypersensitive to oxidants, linking its primary role to redox homeostasis rather than to DNA replication or flagellar motility.

Ref: Prescott's Microbiology, 11th ed., Chapter 7: Glutathione and Oxidative Stress Protection in Bacteria.