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#hydrolysis

10 public questions tagged with this topic.

Which alcohol is produced when ethyl magnesium bromide reacts with acetaldehyde followed by hydrolysis?

Ethyl magnesium bromide (CH₃CH₂MgBr) reacts with acetaldehyde (CH₃CHO), adding the ethyl group to the carbonyl, yielding butan-2-ol (CH₃CH(OH)CH₂CH₃) after hydrolysis.

Ref: NCERT Class 12 Chemistry > Chapter 7: Alcohols, Phenols and Ethers > Topic: Preparation of Phenols and Ethers - Williamson and Other Methods

Driving force for DNA synthesis is hydrolysis of

Incorporation of dNTP involves formation of phosphodiester bond between 3' OH of primer and α-phosphate of nucleotide, releasing inorganic pyrophosphate. Under cellular conditions, subsequent hydrolysis of pyrophosphate into two orthophosphates by inorganic pyrophosphatase yields substantial free energy change around -33 kJ/mol, pulling polymerization equilibrium forward and making overall reaction essentially irreversible. ATP hydrolysis powers helicase and ligase steps but not polymerase condensation itself. Continuous removal of pyrophosphate prevents reverse pyrophosphorolysis reaction, ensuring high processivity and directionality of chain elongation while providing thermodynamic driving force independent of ATP supply.

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 5: Driving Force for DNA Synthesis is Hydrolysis of Pyrophosphate

The energy released by ATP hydrolysis is primarily used for:

Driving endergonic reactions is the accurate answer because it correctly identifies the biological function or role described in this question. In Bioenergetics, understanding the specific functions of molecules, enzymes, or structures is fundamental. Driving endergonic reactions fulfills this particular biological role through its specific structural properties, biochemical activity, or physiological mechanism. The other options (Maintaining osmotic balance, Storing excess energy in cells, and DNA replication) serve different biological functions or are associated with other processes, pathways, or structural roles within the cell or organism.

Ref: Campbell Biology, Urry et al., 12th Ed.

The lifetime of a peptide bond is approximately:

1000 years is the scientifically accurate answer to this question. Within the study of Peptide and Ramachandran plo, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of 1000 years directly address what is being asked. Among the other options, 1 year, 10,000 years, and 1 million years do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Campbell Biology, Urry et al., 12th Ed.

β-thioglucosidases (myrosinases) hydrolyze:

Correct option is C, Glucosinolates. In PSS- Sec G- Sec metabolites problems like this, the accurate statement is Glucosinolates; the others are common mix-ups. Not these: A) Alkaloids; B) Terpenoids; D) Phenolics. Tissue transport questions usually turn on xylem vs phloem, living vs dead cells, or source vs sink.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.