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#treatment

2 public questions tagged with this topic.

Immunological Applications

Which of the following is an example of therapeutic monoclonal antibody?

Rituximab illustrates first FDA approved chimeric therapeutic monoclonal antibody transforming oncology and autoimmunity management. Engineering used recombinant DNA swapping murine variable regions conferring CD20 specificity onto human IgG1 kappa constant domains, approximately 65 percent human to reduce HAMA human anti-mouse antibody response while retaining effector function. Constant region Fc glycosylated at Asn297 in CH2 domain with fucosylated biantennary glycan necessary for high affinity interaction with FcγRIIIa on natural killer cells and macrophages. Production in Chinese hamster

Ref: FDA Monoclonal antibody Rituximab label; Nature Rev Drug Discov 2018 17:31 chimeric anti-CD20 B depletion lymphoma.

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Immunological Applications

Which of the following is an example of therapeutic monoclonal antibody?

Cetuximab chimeric IgG1 mAb 152 kDa murine variable regions grafted human constant domains preserving antigen binding specificity reducing HAMA response versus purely murine. Target EGFR HER1 encoded EGFR 7p11.2 overexpressed colorectal lung HNSCC via amplification autocrine loop. Physiologic ligands EGF TGF-alpha amphiregulin bind ECD III inducing conformational change exposing dimerization arm domain II allowing homo hetero-dimerization HER2 HER3 autophosphorylation tyrosines Tyr1068 Tyr1086 Tyr1173 recruiting adaptors Grb2 Shc activating KRAS GTP exchange GDP GTP triggering BRAF MEK ERK pro

Ref: FDA Erbitux Cetuximab EGFR MOA Label; NEJM Cetuximab Colorectal KRAS Wild-type 2008 Van Cutsem; Janeway EGFR Antibody Therapy ADCC Mechanism.

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