CSIR UGC June 2012

CSIR UGC June 2012

Double stranded DNA replicates in a semi –conservative manner. In an in vitro DNA synthesis reaction , dideoxy CTP and ,

In double stranded dna replicates in a semi, 1 represents established outcome. functional assays confirms option A (1) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

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CSIR UGC June 2012

A protein contains 2 Trp and 4 Tyr residues. The molecular mass of the protein is 17000 D and that of Trp and Tyr are 20

In a protein contains 2 trp and 4, 1.0 represents established outcome. functional assays confirms option B (1.0) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.

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CSIR UGC June 2012

For 5’ end labelling of DNA, the following reaction are carried out sequentially as indicated 5’ dephosphorylated DNA →

In  for 5’ end labelling of dna,, NH4+ ion inhibits T4PNK, therefore should not be present in represents established outcome. functional assays confirms option B (NH4+ ion inhibits T4PNK, there) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

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CSIR UGC June 2012

For the generation of transgenic plant in crop improvement, one important regulatory gene ‘X’ was overexpressed in a cro

for the generation of transgenic plant in operates via Gene silencing effect represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Taiz, Zeiger, Møller and Murphy, Plant Physiology and Development, 6th Edition, chapter on phytohormone biosynthesis and sucrose metabolism, covers ABA enzymes, SPS regulation and stress signaling pathways in higher plants, published by Oxford University Press.

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CSIR UGC June 2012

If one wishes to design a microarray chip for whole genome expression analysis of an eukaryotic system, which region of

Analysis of if one wishes to design a microarray shows 3’ region from coding DNA sequence (CDS) and 3’ untranslated represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

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CSIR UGC June 2012

Four different receptors viz, A, B, C and D bind to same ligand ‘X’. In order to determine when receptors has the highes

In four different receptors viz, a, b, c, C represents established outcome. functional assays confirms option C (C) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.

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CSIR UGC June 2012

In Radio Immuno Assay (RIA) for glucocorticoid hormone, glucocorticoid (tritiated) is added to RIA cocktail. When the am

in radio immuno assay (ria) for glucocorticoid operates via B and C represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Murphy and Weaver, Janeway's Immunobiology, 10th Edition, section on adaptive immunity and immunoassays, details competitive ELISA design, antibody-antigen kinetics and detection methods for quantitative immunology, published by W.W. Norton.

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CSIR UGC June 2012

The Scatter plot of growth rate and growth yield for 100 random environment isolates of bacteria is shown below Which of

Analysis of the scatter plot of growth rate and shows High growth rate cannot be accompanied by high growth yield. represents established outcome. functional assays supports option D, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Madigan, Bender, Buckley and Stahl, Brock Biology of Microorganisms, 16th Edition, chapter on microbial symbioses and nitrogen fixation, describes Rhizobium-legume interactions, nodule physiology and nitrogenase oxygen sensitivity, published by Pearson.

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CSIR UGC June 2012

Stem cells are widely used for their regenerative property and capacity to differentiate into different lineages. A pers

stem cells are widely used for their operates via Transforming skin cells from patient into iPS cells and usin represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

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CSIR UGC June 2012

Which of the following curve correctly represents the antibiotic production by Streptomyces species?

In which of the following curve correctly represents, 2 represents established outcome. functional assays confirms option B (2) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

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CSIR UGC June 2012

Northern elephant seal had been reduced to about 20 in 1800s. Biologist studied variation in protein in the species. The

Analysis of northern elephant seal had been reduced to shows population bottle neck and genetic drift represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

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CSIR UGC June 2012

Biologist randomly sampled about 300 insects from a newly found island. The distribution of their abundance in the sampl

Analysis of biologist randomly sampled about 300 insects from shows All species from the island may not be represented in the sa represents established outcome. functional assays supports option D, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

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