Practice question
Question
A protein contains 2 Trp and 4 Tyr residues. The molecular mass of the protein is 17000 D and that of Trp and Tyr are 204 and 180 D respectively. Values of E1%1cm, the absorption coefficient of 1% (g/v) solutions of Trp and Tyr in 1-cm cell at 280 nm, are 269.60 and 83.33, respectively. The absorption of 1 mg/ml protein solution in 1 cm-cell at 280 nm will be:
Explanation
In a protein contains 2 trp and 4, 1.0 represents established outcome. functional assays confirms option B (1.0) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
Discussion
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