Practice question
Question
Double stranded DNA replicates in a semi –conservative manner. In an in vitro DNA synthesis reaction , dideoxy CTP and , dideoxy CMP were individually added in excess (in separate reaction tubes) in addition to dNTPs and other necessary reagents. Rate of DNA Synthesis was measured by incorporation of 3H-thymidine. The four graphs drawn below represent the rate of DNA synthesis in two separate reaction tubes. Which of the following graphs represents the expected data?
Explanation
In double stranded dna replicates in a semi, 1 represents established outcome. functional assays confirms option A (1) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
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