GATE Biotechnology February 2015

GATE Biotechnology February 2015

Plasmid DNA (0.5 µ g) containing an ampicillin resistance marker was added to 200 µ 1 of competent cells. The transforme

Calculation based on standard molecular principles yields as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Watson et al., Molecular Biology of the Gene, chapter on plasmid dna 0 5 g containing, details DNA replication, transcription, repair mechanisms, recombinant DNA technology and sequence analysis methods with experimental evidence, published by Pearson Education.

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GATE Biotechnology February 2015

Oxygen transfer was measured in a stirred tank bicreactor using dynamic method. The dissolved oxygen tension was found t

Calculation based on standard bioprocess principles yields 0.073 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Shuler and Kargi, Bioprocess Engineering: Basic Concepts, chapter on oxygen transfer was measured in a, presents kinetics, material balances, reactor design, scale-up criteria and productivity calculations for biotechnological processes, published by Prentice Hall.

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GATE Biotechnology February 2015

Samples of bacterial culture taken at 5 PM and then the next day at 5 AM were found to have 10^4 and 10^7 cells-mL^-1. r

Calculation based on standard cell principles yields 1.2 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Alberts et al., Molecular Biology of the Cell, section on samples of bacterial culture taken at, discusses cell structure, membrane organization, cell cycle regulation and intracellular signaling with detailed illustrations, published by Garland Science.

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GATE Biotechnology February 2015

Saccharonores cerevisiae produces ethanol by fermentation. The theoretical yield of ethanol from 2.5 g of glucose is ...

Calculation based on standard biochemistry principles yields 1.28 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, section on saccharonores cerevisiae produces ethanol by fermentation, explains metabolic pathways, enzyme mechanisms, structural biochemistry and thermodynamic principles governing biomolecular transformations, published by W. H. Freeman and Comp

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GATE Biotechnology February 2015

A synchronous culture containing 1.8 x 10^5 monkey kidney cells was seeded into three identical flasks. The doubling tim

Calculation based on standard cell principles yields 2 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Alberts et al., Molecular Biology of the Cell, section on a synchronous culture containing 1 8, discusses cell structure, membrane organization, cell cycle regulation and intracellular signaling with detailed illustrations, published by Garland Science.

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GATE Biotechnology February 2015

A heterozygous tall plant (Tt) was crossed with a homozygous dwarf plant (tt). The resultant seeds were collected. If fi

Calculation based on standard genetics principles yields 31.25 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

Ref: Pierce, Genetics: A Conceptual Approach, chapter on a heterozygous tall plant tt was, covers Mendelian inheritance, population genetics, allele frequencies, penetrance and quantitative genetics with worked examples, published by W. H. Freeman.

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GATE Biotechnology February 2015

The following question presents a sentence, part of which is underlined Beneath the sentence you find four ways of phras

ranks as one of the leading causes of death is correct because it matches established general principles where ranks as one of the leading causes of death represents the functional mechanism. Alternatives such as rank as one of the leading causes of death, has the rank of one of the

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on the following question presents a sentence, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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GATE Biotechnology February 2015

If logx (5/7) = -1/3, then the value of x is

343/125 is correct because it matches established general principles where 343/125 represents the functional mechanism. Alternatives such as 125/343, -25/49 contradict experimental evidence and standard textbook descriptions. This reasoning aligns with standard definitions, laboratory observations and quantitative analysis commonly taught in biotechnology curricula.

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on if logx 5 7 1 3, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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GATE Biotechnology February 2015

Choose the appropriate word/phrase. out of the four options given below, to complete the following sentence: Frogs

croak is correct because it matches established general principles where croak represents the functional mechanism. Alternatives such as roar, hiss contradict experimental evidence and standard textbook descriptions. This reasoning aligns with standard definitions, laboratory observations and quantitative analysis commonly taught in biotechnology curricula.

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on choose the appropriate word phrase out, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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GATE Biotechnology February 2015

Match the reagents in Group I with their preferred cleavage sites in Group II Group I (Agent) Group II (Cleavage Site) P

P-1. Q-3. R-5. S-4 is correct because it matches established general principles where p-1. q-3. r-5. s-4 represents the functional mechanism. Alternatives such as P-2. Q-3.R-1-. S-4, P-1. Q-2, R-5. S-4 contradict experimental evidence and standard textbook descriptions.

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on match the reagents in group i, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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GATE Biotechnology February 2015

Production of monoclonal antibodies by hybxidoma technology requires

splenocytes is correct because it matches established general principles where splenocytes represents the functional mechanism. Alternatives such as osteocytes, heparocytes contradict experimental evidence and standard textbook descriptions. This reasoning aligns with standard definitions, laboratory observations and quantitative analysis commonly taught in biotechnology curricula.

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on production of monoclonal antibodies by hybxidoma, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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GATE Biotechnology February 2015

Which one of the following organises is used for the determination of phenol coefficient of a disinfectant?

Salmonella typhi is correct because it matches established general principles where salmonella typhi represents the functional mechanism. Alternatives such as Escherichia coli, Candida albicans contradict experimental evidence and standard textbook descriptions.

Ref: Griffiths et al., An Introduction to Genetic Analysis, section on which one of the following organises, provides foundational concepts, mathematical derivations, probability calculations and analytical methods for biotechnology problem solving, published by W. H. Freeman.

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