CSIR UGC june 2018

CSIR UGC june 2018

Which one of the following set of essential components are required for Sanger method of DNA sequencing in a required bu

In which one of the following set of, template, primer, 4 dNTPs, 4 ddNTPs, polymerase, Mg²⁺/Tris; ddNTPs terminate chains. dideoxy principle confirms option A (DNA template, a primer, 4 deox) as correct, while alternatives misstate ligase requirement, contradicting textbook data.

Ref: Primrose and Twyman, Principles of Gene Manipulation and Genomics, 8th Edition, chapter on gene cloning, Sanger sequencing and expression vectors, covers primer design, dideoxy termination and recombinant protein purification strategies, published by Wiley-Blackwell.

View Details

CSIR UGC june 2018

A certain protein has been assumed to play an indispensable role in the survival of an intracellular parasite inside the

Analysis of a certain protein has been assumed to shows Treat the parasite-infected host cells with an inhibitor of represents established outcome. functional assays supports option A, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.

View Details

CSIR UGC june 2018

In order to visualize the intracellular organization of a cell, one can utilize various microscopy-based techniques. The

in order to visualize the intracellular organization operates via A, B, C, D, G represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: CSIR-NET General Aptitude preparation module, quantitative reasoning section covering number theory, combinatorics and logical deduction, explains divisibility tests, sequence progression and probability calculations used for Part A problem solving, compiled from national examination resources.

View Details

CSIR UGC june 2018

A researcher attempted to clone two genes (X and Y) independently in a plasmid vector for over expression and purificati

a researcher attempted to clone two genes operates via A, C and D represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

View Details

CSIR UGC june 2018

From the following statements, A. Surface plasmon resonance can be used to determine binding constants only in the range

from the following statements, a. surface plasmon operates via C, D represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Nelson and Cox, Lehninger Principles of Biochemistry, 8th Edition, chapter on enzyme kinetics, protein structure and metabolic pathways, details active site chemistry, cofactor requirements and allosteric regulation supporting biochemical interpretations, published by W.H. Freeman.

View Details

CSIR UGC june 2018

Sub-cellular fractionation-based assays have been used to identify various organelles in the mammalian cells. In order t

sub-cellular fractionation-based assays have been used to operates via use of organelle specific fluorescent probes followed by mic represents established outcome. functional assays validates option B. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Alberts et al., Molecular Biology of the Cell, 7th Edition, chapter on membrane structure and intercellular junctions, explains plasmodesmata organization, cytoskeletal interactions and organelle compartmentalization in eukaryotic cells, published by W. W. Norton and Garland Science.

View Details

CSIR UGC june 2018

Three students (P, Q, R) in a research lab were trying to identify proteins that interact with a transcription factor X.

Analysis of three students (p, q, r) in a shows ( i ), (ii) and (iv) represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

View Details

CSIR UGC june 2018

In order to detect minor variations in antigen concentration, the following procedures were suggested. Which one will li

in order to detect minor variations in operates via Preincubate antigen with fixed amount of antibody → add to a represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Murphy and Weaver, Janeway's Immunobiology, 10th Edition, section on adaptive immunity and immunoassays, details competitive ELISA design, antibody-antigen kinetics and detection methods for quantitative immunology, published by W.W. Norton.

View Details

CSIR UGC june 2018

A 1257 bp genomic DNA sequence of a prokaryotic gene was cloned under a strong constitutive promoter along with a suitab

a 1257 bp genomic dna sequence of operates via A and B represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

Ref: Primrose and Twyman, Principles of Gene Manipulation and Genomics, 8th Edition, chapter on gene cloning, Sanger sequencing and expression vectors, covers primer design, dideoxy termination and recombinant protein purification strategies, published by Wiley-Blackwell.

View Details

CSIR UGC june 2018

Following table contains some of the generalizations of evolutionary biology: Which of the following is correct match be

Analysis of following table contains some of the generalizations shows A - ( i ), B - (iii), C - (iv), D - (ii) represents established outcome. functional assays supports option B, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Pierce, Genetics: A Conceptual Approach, 7th Edition, chapter on transmission genetics, linkage and population genetics, explains allele segregation, Hardy-Weinberg principles and epistatic interactions underlying inheritance patterns, published by Macmillan Learning.

View Details

CSIR UGC june 2018

Following are key points about the effect of genetic drift: A. Genetic drift is significant in small populations. B. Gen

Analysis of following are key points about the effect shows A, C and D represents established outcome. functional assays supports option D, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Begon, Townsend and Harper, Ecology: From Individuals to Ecosystems, 4th Edition, chapter on community ecology and ecosystem energetics, explains historical contributions of Elton, Tansley, Lotka and Lindeman to trophic dynamics and ecosystem theory, published by Wiley-Blackwell.

View Details

CSIR UGC june 2018

Given are some statements with reference to the use of genes in plant molecular systematics. A. mtDNA are not preferred

Analysis of given are some statements with reference to shows B and D only represents established outcome. functional assays supports option D, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

Ref: Watson et al., Molecular Biology of the Gene, 7th Edition, section on DNA replication, transcription and repair mechanisms, describes polymerase fidelity, foot-printing principles and gene regulation networks relevant to molecular assays, published by Pearson Education.

View Details