Practice question

MCQ

Question

In order to detect minor variations in antigen concentration, the following procedures were suggested. Which one will likely be the best option?

Options

Choose one · Correct answer highlighted

Antigen coated microtitre well → add antibody → add enzyme conjugated secondary antibody → add substrate and measure colour. Antibody coated microtitre well → add antigen → add enzyme conjugated secondary antibody → add substrate and measure colour. Preincubate antigen with fixed amount of antibody → add to antigen coated well → add enzyme conjugated secondary antibody → add substrate and measure colour. Correct Preincubate antigen with fixed amount of antibody → add to antibody coated well → add enzyme conjugated secondary antibody → add substrate and measure colour.

Explanation

in order to detect minor variations in operates via Preincubate antigen with fixed amount of antibody → add to a represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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