Practice question
Question
Which one of the following set of essential components are required for Sanger method of DNA sequencing in a required buffer containing MgCl2 and Tris-HCl?
Explanation
In which one of the following set of, template, primer, 4 dNTPs, 4 ddNTPs, polymerase, Mg²⁺/Tris; ddNTPs terminate chains. dideoxy principle confirms option A (DNA template, a primer, 4 deox) as correct, while alternatives misstate ligase requirement, contradicting textbook data.
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