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#suspension cells

2 public questions tagged with this topic.

Animal Tissue Culture 2

Cells that grow freely in suspension include:

Cells that proliferate freely without attachment are predominantly of hematopoietic origin including T lymphocytes, B lymphocytes, monocytes, neutrophils, erythroid progenitors, and leukemic lines derived from them such as Jurkat, Raji, THP-1. These cells physiologically exist in circulation or bone marrow where immobilization is unnecessary and survival signals arise from cytokines activating JAK-STAT pathways rather than integrin-ECM engagement. In culture they grow as spherical single cells or small clusters suspended in medium, requiring agitation only for oxygenation in large scale stirre

Ref: Freshney Ch.14 Hematopoietic suspension growth; Lodish MBoC Ch.24 Blood cells suspension culture cytokine dependence.

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Animal Tissue Culture 2

Which cells do NOT require trypsin for passaging?

Adherent cell types such as fibroblasts, epithelial, endothelial, and myoblasts develop integrin-mediated focal adhesions and cadherin-based cell-cell contacts requiring proteolytic cleavage for detachment. Trypsin-EDTA treatment disrupts these connections enabling replating. Hematopoietic lineage cells including lymphocytes, monocytes, erythroblasts, mast cells, and numerous leukemia derivatives such as Jurkat, K562, HL-60 naturally grow in suspension, circulating in bloodstream and bone marrow without organized extracellular matrix attachment. They rely on cytokines like IL-2, IL-3, GM-CSF f

Ref: Freshney Ch.14 Suspension vs adherent cultures; Abbas Cellular and Molecular Immunology 10th Ed hematopoietic cells suspension growth.

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