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#ligand concentration

2 public questions tagged with this topic.

Cell Signaling Fundamental PrinciplesI

Ligand concentration can be encoded as

pulse amplitude and duration, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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Cell Signaling Fundamental Principles

At half saturation, ligand concentration equals

When half of the available receptors are occupied, fractional saturation equals 0.5. Substitution into the binding equation shows that the free ligand concentration at this point must equal the dissociation constant Kd. Measurement of the ligand concentration that produces half-maximal occupancy therefore yields Kd directly, provided nonspecific binding has been subtracted and equilibrium has been reached.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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