Which protein facilitates vesicle uncoating after transport?
Temporal control coat association ensures vesicle budding but permits subsequent fusion because SNAREs hidden under coat cannot engage target. After detachment coat must be shed within seconds exposing v-SNAREs for tethering. Distinct uncoating mechanisms evolved: COPII shedding triggered by Sar1 GTP hydrolysis stimulated by Sec23 GAP plus Sec13-31 destabilization; COPI shedding by ARF GAP1/2 ASAP stimulating ARF1 hydrolysis plus curvature mismatch. Clathrin uncoating for AP2 and AP1/GGA vesicles requires chaperone system unique: DnaJ co-chaperone auxilin 1/2 specifically recognizes assembled
Ref: Sousa & Lafer, Traffic 2015: Hsc70 and auxilin mediate clathrin uncoating.