Which of these is used to analyze gross chromosomal rearrangements like translocations?
Multiplex FISH, or M-FISH, extends conventional FISH by using combinatorial labeling of whole-chromosome painting probes with distinct fluorochrome combinations, generating a unique spectral signature for each chromosome. This 24-color karyotyping allows simultaneous visualization of all chromosomes in different hues. Gross abnormalities such as reciprocal translocations, dicentric chromosomes, insertions, and complex rearrangements, which are difficult to resolve with single-locus probes or DNA microarrays and are not detectable by RAPD or RT-PCR, become readily identifiable as color junction
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
