Enzymes commonly used for tissue disaggregation include:
Conversion of solid animal tissue into single cell suspension requires enzymatic disaggregation targeting extracellular matrix and intercellular junctions. Trypsin, serine protease from porcine pancreas, hydrolyzes peptide bonds on carboxyl side of basic residues lysine and arginine abundant in adhesion proteins fibronectin, laminin, and cadherin extracellular domains. Collagenase, derived from Clostridium histolyticum, specifically cleaves triple-helical native collagen rich in proline-hydroxyproline-glycine repeats found in connective tissue, basement membrane, and cartilage, requiring calci
Ref: Freshney Culture of Animal Cells Ch.11 Primary Culture Trypsin-Collagenase; Lodish Molecular Cell Biology 8th Ed ECM degradation enzymes.