Ti plasmid vector commonly used for antisense constructs is:
Transformation of tomato for delayed ripening relies on Agrobacterium tumefaciens binary vector system exemplified by pBIN19. Native Ti plasmid C58 type is ~200 kb containing T-DNA bordered by 25 bp imperfect direct repeats and vir regulon encoding VirA sensor kinase, VirG response regulator, VirD1/D2 endonuclease, VirB T4SS pilus and VirE2 single-stranded DNA binding protein. To facilitate cloning, large plasmid split into helper Ti plasmid disarmed lacking oncogenes iaaM, iaaH, ipt responsible for auxin cytokinin overproduction causing crown gall, retaining vir genes, and small shuttle vecto
Ref: Bevan M 1984 Nucleic Acids Res 12:8711 pBIN19; Gelvin SB Microbiol Mol Biol Rev Agrobacterium Ti.