Which of the following microscopy techniques uses Moiré fringes for super-resolution imaging?
Structured Illumination Microscopy enhances resolution by projecting patterned light onto sample, generating Moiré fringes where illumination and specimen spatial frequencies interfere. These interference patterns encode high-frequency structural information normally beyond diffraction limit into detectable low-frequency moiré. Multiple images acquired with shifted and rotated gratings are mathematically reconstructed to double lateral resolution to ~100 nm and improve axial resolution. SIM requires only conventional fluorophores, low laser power, and works with live cells, making it valuable
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.