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#shikimate pathway

4 public questions tagged with this topic.

The shikimate pathway is involved in synthesis of:

Shikimate pathway bridges central metabolism and aromatic compound biosynthesis unique to plants, fungi, and bacteria, absent in animals. Starting substrates phosphoenolpyruvate from glycolysis and erythrose-4-phosphate from pentose phosphate pathway undergo condensation by DAHP synthase initiating seven enzymatic steps producing 3-dehydroquinate, dehydroshikimate, shikimate, shikimate-3-phosphate, EPSP, and chorismate. Chorismate branches to prephenate leading to phenylalanine and tyrosine via arogenate pathway, and to anthranilate leading to tryptophan. Aromatic amino acids contribute to pro

Ref: Herrmann & Weaver Annu Rev Plant Physiol 1999 shikimate pathway; NCBI NBK22426; Taiz & Zeiger Ch 13; https://www.ncbi.nlm.nih.gov/books/NBK22426/

Glyphosate inhibits which enzyme?

Glyphosate N-phosphonomethyl glycine inhibits key enzyme of aromatic amino acid pathway. 5-enolpyruvylshikimate-3-phosphate synthase normally condenses shikimate-3-phosphate and phosphoenolpyruvate to EPSP in chloroplast stroma, penultimate step of shikimate pathway. Glyphosate mimics PEP, occupying PEP binding site in EPSPS-S3P complex forming stable dead-end complex preventing enolpyruvyl transfer. Plants cannot synthesize chorismate, precursor of phenylalanine, tyrosine, tryptophan, causing accumulation of shikimate, depletion of aromatic amino acids, cessation of protein synthesis, and dea

Ref: Steinrücken Biochem Biophys Res Commun 1980 glyphosate EPSPS; Funke PNAS 2006 Roundup Ready molecular basis; NCBI NBK22387; https://www.pnas.org/doi/10.1073/pnas.0603638103

Glyphosate inhibits which pathway?

Answer: B) Shikimate. For PLANT SEC METABOLITES, remember the key idea and you’ll land on this option; the distractors usually swap cause and effect or confuse similar terms. Avoid: A) MEP; C) Malonate; D) MVA. Name the process first, then pick the option that describes it — don’t reverse cause and effect.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.

Phenolics are synthesized mainly via:

B fits best: Shikimic acid pathway. Keep the PLANT SEC METABOLITES basics straight and Shikimic acid pathway stands out as the precise answer. Avoid: A) Glycolysis; C) TCA cycle; D) PPP. Light and flowering items often hinge on which photoreceptor or day-length rule is in play.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.