Cells secreting DIF-1:
DIF-1 biosynthetic genes stlB encoding polyketide synthase and dmtA encoding desmethyl DIF-1 methyltransferase are preferentially transcribed in prespore region under cAMP and PKA induction. Prespore cells therefore synthesize chlorinated polyketide and release it, where it diffuses to anterior prestalk population acting paracrinally. Prestalk cells contain receptors and downstream STAT machinery but low biosynthetic capacity, functioning as responders. Stalk cells and mature spores show negligible synthesis. This spatial separation creates feedback loop ensuring prespore majority controls sta
Ref: Development Journal, DIF-1 biosynthesis genes stlB/dmtA in prespore cells - feedback regulation of stalk proportion.