In a pedigree analysis using X-linked STRs, recombinant chromosomes were found in:
X-linked short tandem repeat analysis examines transmission of maternal and paternal X chromosomes to sons and daughters. Recombinant X chromosomes result from crossing over in maternal meiosis, producing new allelic combinations of linked STR loci compared to parental haplotypes. Non-recombinant offspring retain intact parental haplotypes. Identification relies on comparing haplotype phases across multiple STR markers. In this pedigree, individuals showing shuffled allele patterns at adjacent X-linked loci represent recombination events. Recognizing recombinants is crucial for accurate linkag
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.