Which method provides more accurate DNA quantification on gels?
Accurate DNA quantification on gels requires measurement of fluorescence intensity proportional to DNA mass. Visual estimation by eye comparing to ladder is subjective and semiquantitative. Densitometry employs gel documentation systems with CCD cameras and software to integrate band pixel intensity, generating quantitative curves calibrated against standards. This provides objective measurement of amount and molecular weight. Tracking dye comparison monitors migration, not mass. Heat mapping is not standard. Therefore densitometric analysis offers superior precision and reproducibility for DN
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
