Skip to content
Login Register

#protein production

4 public questions tagged with this topic.

Animal Tissue Culture 3

CHO cells are extensively used for:

Logarithmic or exponential phase follows lag and is characterized by maximal and constant specific growth rate where population doubles at regular intervals, producing straight line on semi-logarithmic plot of cell number versus time. Birth rate greatly exceeds death rate. Cellular activities peak including DNA replication by DNA polymerases alpha and delta, rRNA transcription, protein synthesis, high glucose consumption via aerobic glycolysis producing lactate, and oxygen uptake by mitochondria. For most continuous lines generation time 18-24 hours though HeLa approximately 20 hours. Contact

Ref: Freshney Ch.13 Log phase exponential growth; Lodish MBoC Ch.12 Cell cycle exponential proliferation and growth curve.

View Details
Gene Therapy

Factor VIII recombinant protein is commonly produced in:

Coagulation factor VIII complex multidomain glycoprotein organized signal peptide 19 residues followed domains A1 a1 A2 a2 B a3 A3 C1 C2 totaling 2351 aa 280 kDa requiring extensive co-translational post-translational processing secretory pathway. Nascent polypeptide translocates Sec61 translocon ER lumen signal peptidase cleaves signal oligosaccharyltransferase adds high-mannose oligosaccharides 25 asparagine X Ser Thr motifs protein disulfide isomerase forms 8 disulfide bonds calnexin calreticulin cycle glucosidase II monitors folding peptidyl prolyl isomerase assists. Golgi further trims ma

Ref: Blood Factor VIII CHO Production Kaufman 1988; FDA Recombinant Factor VIII Manufacturing Guidelines; Alberts Protein Glycosylation Mammalian Cells Chap 15.

View Details