Which of the following proteins uses a GPI anchor to attach to the membrane?
Classification of lipid-anchored proteins includes GPI anchor addition in endoplasmic reticulum lumen via multi-step pathway conserved across eukaryotes. GPI precursor synthesis starts on cytoplasmic face adding N-acetylglucosamine to phosphatidylinositol by PIG-A, deacetylation by PIG-L, flipping to luminal side by flippase, addition of three mannose residues from dolichol-phosphate-mannose by PIG-M PIG-V PIG-B, addition of phosphoethanolamine by PIG-O PIG-F, final transfer en bloc to C-terminal GPI signal peptide cleavage site containing omega site small residues by GPI transamidase comprisi
Ref: Mayor and Riezman, Sorting GPI-Anchored Proteins, Nature Reviews Mol Cell Biol 2004.
