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#polyadenylation

3 public questions tagged with this topic.

Processing of mRNA, rRNA, tRNA

Polyadenylation occurs at which end of mRNA?

Polyadenylation of eukaryotic messenger RNA occurs exclusively at three prime end, downstream of AAUAAA signal where nascent RNA is cleaved by endonuclease CPSF73. Polyadenylate polymerase PAP adds continuous stretch of adenine residues to new three hydroxyl group in template-independent manner using ATP. Location exclusively three prime is critical for defining messenger RNA orientation, promoting nuclear export, protecting against three prime to five prime exosome decay, and enhancing translation initiation via interaction between polyadenine binding protein PABPC and eIF4G that circularizes mRNA. Replication-dependent histone mRNAs are exception lacking polyadenylation.

Ref: Lodish et al., Molecular Cell Biology, 9th ed., Chapter 10: Polyadenylation at 3' end; Alberts 7th ed., Poly(A) tail addition site

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Acid, Base, pH

Eukaryotic mRNAs are generally

monocistronic is the scientifically accurate answer to this question. Within the study of Acid, Base, pH, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of monocistronic directly address what is being asked. Among the other options, polycistronic, overlapping, and non-coding do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

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