Which statement is FALSE about poly(A) profiling?
Poly(A) profiling is a 3′-end focused transcriptomic method that captures and sequences regions adjacent to polyadenylation sites. By using oligo(dT)-based enrichment and sequencing of the 3′ termini, it identifies alternative polyadenylation events, novel transcripts defined by distinct 3′ ends, and can reveal 3′ UTR alterations associated with gene fusions. Stating that it analyzes 5′ ends misrepresents its design, since 5′ cap analysis is performed by CAGE, not poly(A) profiling. Therefore the claim of 5′ end analysis is inconsistent with its mechanistic foundation and primary applications.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
