Ti plasmid vector commonly used for antisense constructs is:
Transformation of tomato for delayed ripening relies on Agrobacterium tumefaciens binary vector system exemplified by pBIN19. Native Ti plasmid C58 type is ~200 kb containing T-DNA bordered by 25 bp imperfect direct repeats and vir regulon encoding VirA sensor kinase, VirG response regulator, VirD1/D2 endonuclease, VirB T4SS pilus and VirE2 single-stranded DNA binding protein. To facilitate cloning, large plasmid split into helper Ti plasmid disarmed lacking oncogenes iaaM, iaaH, ipt responsible for auxin cytokinin overproduction causing crown gall, retaining vir genes, and small shuttle vector pBIN19 carrying replication origins ColE1 for E. coli and RK2 for Agrobacterium, kanamycin resistance nptIII for bacterial selection, left and right borders, multiple cloning site with lacZ alpha for blue-white screening adjacent to CaMV35S promoter and nos terminator, plus plant selectable nptII driven by nos promoter and marker. Antisense PG cDNA cloned in reverse orientation between promoter and terminator mobilized to Agrobacterium via triparental mating, acetosyringone induced vir expression nicked borders, T-strand transferred integrated via nonhomologous end joining semi-randomly. pBIN19 advantage over pUC19, pBR322 lacking T-DNA and plant signals.
Ref: Bevan M 1984 Nucleic Acids Res 12:8711 pBIN19; Gelvin SB Microbiol Mol Biol Rev Agrobacterium Ti.