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#plant pathology

5 public questions tagged with this topic.

Which disease was introduced to India through plant introduction?

Historical evidence documents inadvertent pathogen establishment via planting material. Coffee rust caused by Hemileia vastatrix, basidiomycete producing orange urediniospores on lower leaf surface causing chlorosis and premature defoliation, originated in Sri Lanka then Ceylon and entered India in 1876 through infected seedlings imported for plantation expansion. Epidemic destroyed Arabica areas in Western Ghats, forcing shift to robusta Coffea canephora which carries resistance SH genes. Similar introductions: late blight Phytophthora infestans through Europe, bunchy top virus of banana via suckers. Mycelium survival in vegetative propagules escapes visual inspection, emphasizing need for molecular diagnostics, ELISA and post-entry isolation growing. Lesson reinforced need for stringent phytosanitary certification before cultivar release. This understanding supports competitive exam preparation for NEET, GATE and CSIR NET concepts linking genotype with phenotype through molecular pathways involving transcription factors, hormones and metabolic enzymes that regulate development, adaptation and reproductive biology in applied breeding programs. Additional insights from genomic studies reveal QTL clusters, epigenetic modifications and protein interactions that influence trait expression under varied agro-climatic conditions and management.

Ref: Indian Coffee Board history rust introduction; NCERT Plant Disease introduction case; Singh BD Disease introduction chapter.

A researcher chooses a method that is simple and fast for TMV resistance detection. Which is best?

Random amplified polymorphic DNA offers rapid, inexpensive screening without requirement for sequence information, radioactive probes or sophisticated instrumentation. Using a single short random primer in a standard PCR, numerous loci are amplified simultaneously, producing easily scorable fingerprints on agarose gels within hours. For preliminary disease resistance screening such as Tobacco mosaic virus, quick identification of linked fragments is advantageous. RFLP and AFLP involve laborious digestion, blotting and selective amplification steps, while EST-SSR development requires transcriptome sequencing and primer validation, increasing cost and time.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.