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#plant genetic engineering

5 public questions tagged with this topic.

Transgenic Plant -1

Atrazine resistance is mediated by:

Atrazine belongs to s-triazine class that binds plastoquinone binding niche of photosystem II D1 protein encoded by psbA, interrupting electron flow from QA to QB, generating singlet oxygen and lipid peroxidation causing chlorosis. While psbA Ser264 to Gly mutation confers resistance in weeds, crop tolerance in maize mediated differently via enhanced metabolic detoxification. Glutathione S-transferases especially Tau and Phi classes conjugate triazine chlorine with sulfhydryl group of reduced glutathione producing GS-atrazine conjugate that is transported to vacuole by ABC transporters and fur

Ref: Shimabukuro Plant Physiol 1970 GST atrazine; Kreuz Curr Opin Plant Biol 2006 GST mediated; NCBI NBK22365; PubMed 16495050.

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Transgenic Plant -1

Bialaphos resistance in plants is conferred by:

Bialaphos tripeptide herbicide consists of phosphinothricin plus two alanine residues. In plant tissue peptidases release active phosphinothricin, structural analog of glutamate that inhibits glutamine synthetase essential for assimilation of ammonia into glutamine. Inhibition causes ammonia accumulation, photosynthesis disruption, and rapid cell death. Bacterium Streptomyces hygroscopicus produces bialaphos and protects itself via bar gene encoding phosphinothricin acetyltransferase. Enzyme transfers acetyl group from acetyl-CoA to free amino group of phosphinothricin, producing N-acetyl phos

Ref: Murakami Mol Gen Genet 1986 bar Streptomyces; De Block EMBO J 1987 Basta resistance; NCBI NBK131103 bar function; PubMed 2884102.

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