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#plant cell wall

4 public questions tagged with this topic.

Plant tissue culture 3

Cellulase and pectinase are used for:

Plant cell walls composed of cellulose microfibrils embedded in hemicellulose and pectin matrix prevent direct membrane manipulation. Protoplast technology demands complete wall removal without lysing protoplasm. Cellulase complex from Trichoderma reesei hydrolyzes β-1,4 glycosidic bonds of cellulose, while pectinase or macerozyme from Rhizopus degrades polygalacturonate of middle lamella, loosening intercellular adhesion. Digestion carried out in isoosmotic solution of mannitol or sorbitol at pH 5.4 to 5.8, supplemented with calcium chloride for membrane stabilization, releases spherical prot

Ref: Cocking 1960 protoplast isolation; Bhojwani & Dantu Ch 14 enzymatic; NCBI PMC3248752 cellulase macerozyme protocol; Gamborg & Phillips Methods.

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Plant tissue culture 2

Protoplasts are plant cells that lack:

Protoplasts are plant cells that completely lack cell wall, defined as protoplasm enclosed only by plasma membrane after removal of cellulose hemicellulose and pectin components. Isolation employs enzyme mixture cellulase hydrolyzing beta 1,4 glucan backbone of cellulose microfibrils and pectinase macerozyme dissolving middle lamella pectin crosslinking cells, often adding hemicellulase driselase; incubation performed in osmoticum mannitol sorbitol 0.4-0.6 molar to prevent lysis due to loss of wall counteracting turgor. After digestion spherical protoplasts released from leaf mesophyll or cell

Ref: Cocking 1960 protoplast isolation; NCBI Bookshelf protoplast manual.

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BASICS of PLANT+PYQS

Wall pressure is exerted by:

Pick B: Cell wall on protoplast. Thinking through BASICS of PLANT step by step rules out the lookalikes and leaves Cell wall on protoplast. Skip these: A) Protoplast on wall; C) Vacuole on wall; D) Plasma membrane. For hormones and genes, match the molecule or gene to its real role, not a neighboring pathway.

Ref: Best CSIR NET Plant Physiology books: Master Unit 6 with Taiz & Zeiger and Salisbury & Ross. Crack Part C experimental questions with top textbooks.

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