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#parentage testing

2 public questions tagged with this topic.

Molecular Marker

STRs used in parentage testing differ based on:

Short Tandem Repeats exhibit polymorphism due to variation in number of tandemly repeated core units at a locus. DNA polymerase slippage adds or deletes repeat units during replication, generating alleles differing in length but not necessarily base composition, methylation state or expression level. Parentage testing exploits highly polymorphic allele length variation; child inherits one allele length from each parent. Base composition and epigenetic modifications do not determine STR genotype. Counting repeat number provides quantifiable allelic diversity crucial for forensic identity and kinship resolution.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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Molecular Marker

In a Southern blot analysis for parentage with STR, which offspring match both parents?

Parentage testing by Southern blotting of short tandem repeats relies on principle that offspring must inherit one allele from each parent. True biological offspring display bands matching maternal and paternal alleles, with at least one band shared with each parent. Offspring lacking paternal specific bands or showing novel bands inconsistent with Mendelian inheritance are excluded. In this blot, several progeny exhibit complementary banding patterns containing maternal and paternal specific fragments, confirming parentage, while mismatched profiles indicate exclusion or mutation, essential for pedigree verification.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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