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#molecular weight

5 public questions tagged with this topic.

Electrophoresis

What is the primary factor responsible for DNA separation in electrophoresis?

In electrophoresis, migration depends on charge to mass ratio and molecular sieving. DNA possesses a uniform negative charge due to phosphate backbone, one per nucleotide, resulting in nearly identical charge/mass ratio for all fragments. Consequently, in free solution, all DNA migrates equally. Separation in agarose or polyacrylamide occurs due to frictional resistance of the porous matrix, where longer molecules experience greater retardation. Thus, although labeled as size-based separation, the underlying principle involves differential mobility governed by charge/mass ratio interacting with sieving and frictional forces.

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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Water and Mole Concept

What will be the molarity of 1 mg/mL protein solution if its molecular weight is 10 kDa?

0.1mM is the scientifically accurate answer to this question. Within the study of Water and Mole Concept, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of 0.1mM directly address what is being asked. Among the other options, 1mM, 10mM, and 100mM do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

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Amino Acids Basics

Which amino acid has the highest molecular weight among the standard amino acids?

Tryptophan is the scientifically accurate answer to this question. Within the study of Amino Acids Basics, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Tryptophan directly address what is being asked. Among the other options, Arginine, Leucine, and Phenylalanine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 3

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Amino Acids Structure

Which amino acid has the lowest molecular weight?

Glycine is the scientifically accurate answer to this question. Within the study of Amino_Acids_Structure, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Glycine directly address what is being asked. Among the other options, Alanine, Serine, and Cysteine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Campbell Biology, Urry et al., 12th Ed.

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Amino Acids Structure

Which amino acid has the highest molecular weight?

Tyrosine is the scientifically accurate answer to this question. Within the study of Amino_Acids_Structure, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Tyrosine directly address what is being asked. Among the other options, Tryptophan, Methionine, and Arginine do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.

Ref: Campbell Biology, Urry et al., 12th Ed.

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