What is the function of the Mitotic Exit Network (MEN)?
Mitotic Exit Network enables yeast cells to coordinate chromosome segregation completion with cytokinesis induction, preventing division before genome partitioned. The network is G-protein coupled kinase cascade anchored at spindle pole bodies. GTPase Tem1, kept inactive when spindle misaligned by Kin4-mediated phosphorylation of Bfa1-Bub2 GAP complex, becomes activated as daughter spindle pole body enters bud containing activating factor Lte1, a guanine nucleotide exchange factor like protein that antagonizes Bub2-Bfa1 hydrolysis activity. GTP-bound Tem1 recruits and activates Cdc15 kinase vi
Ref: Bardin & Amon, The Mitotic Exit Network Signaling Cascade, Nat Rev Mol Cell Biol 2001; NCBI, Yeast MEN Pathway Components.