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#microspore culture

2 public questions tagged with this topic.

Plants produced via androgenesis are initially:

Plants produced via androgenesis are initially haploid bearing single chromosome set n derived from meiotic reduction of diploid mother cells into microspores. After meiosis each microspore contains recombined haploid genome; when cultured without fertilization embryogenic divisions maintain this n number, so tissues leaves roots show halved DNA content compared to diploid control confirmed by flow cytometry and chromosome counting revealing for example 7 chromosomes in barley haploid versus 14 diploid. Such haploids exhibit reduced vigor smaller organs small stomatal guard cells and complete

Ref: Kasha & Kao 1970 haploid plants; Maluszynski et al., FAO doubled haploid manual.

Androgenesis involves culture of:

Androgenesis is male gametophyte-mediated haploid production pathway exploiting totipotency of immature pollen. Anthers containing uninucleate microspores at late uninucleate to early binucleate stage are excised and cultured on nutrient media, or isolated microspores are separated from anthers and cultured in liquid medium under stress. Developmental program of microspore shifts from pollen grain maturation toward sporophytic division, forming embryo-like structures or callus that regenerate haploid plantlets with n chromosomes derived entirely from paternal gametic genotype. Process exploits

Ref: Seguí-Simarro JM. Androgenesis review 2010 J Exp Bot; Maluszynski et al. FAO Haploid protocols – anther culture