The micropyle in Drosophila eggs primarily functions to:
Insect eggshell includes thick chorion and inner vitelline membrane impermeable to sperm. A single micropyle acts as exclusive portal, permitting only one sperm to traverse thick envelopes. Upon fertilization, vitelline membrane undergoes rapid crosslinking and hardening mediated by calcium-dependent enzymes, blocking additional sperm passage. The combination of single narrow canal and fast envelope modification provides structural polyspermy prevention. Since Drosophila eggs normally activate independently of fertilization before sperm entry, preventing multiple sperm pronuclei is critical for diploidy, making micropyle function as anatomical safeguard ensuring monospermic fertilization essential.
Ref: NCBI Bookshelf, Developmental Biology: Fertilization - polyspermy prevention in insects.