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#metabolite production

2 public questions tagged with this topic.

Plant tissue culture 1

Increase in secondary metabolites in cell culture is commonly achieved by:

Increasing secondary metabolite accumulation in plant cell cultures is commonly achieved by supplementation with elicitors after achieving adequate biomass. Secondary metabolism is resource costly competing with primary growth for carbon nitrogen and energy, regulated by growth defense trade off controlled by TOR kinase promoting growth versus SnRK1 promoting defense. Under normal culture high auxin low stress conditions flux stays in primary metabolism. Elicitor addition artificially activates jasmonate and salicylate mediated defense transcriptome inducing genes encoding rate limiting enzyme

Ref: Mishra & Ranjan 2008 secondary metabolite; PubMed elicitor methyl jasmonate.

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Plant tissue culture 1

Elicitors enhance secondary metabolite production by:

Elicitors boost secondary metabolite production by mimicking biotic attack triggering plant innate defense signaling and activation of defence related enzymes. Biotic elicitors include fungal cell wall fragments chitin chitosan glucans yeast extract bacterial flagellin; abiotic include jasmonic acid methyl jasmonate salicylic acid heavy metals copper cadmium UV-C ozone. Perception by pattern recognition receptors triggers calcium influx cyclic nucleotide gated channels, production of reactive oxygen species via NADPH oxidase RBOH, activation of mitogen activated protein kinase cascades MPK3 MP

Ref: Ramachandra & Ravishankar Plant Cell Rep 2002 elicitors; Nature Plants secondary metabolism.

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