Skip to content

#IR fingerprint region

1 public question tagged with this topic.

In IR, fingerprint region lies within:

Infrared spectrum divides into functional group region above 1500 cm-1 and fingerprint region below. The fingerprint region, commonly cited as approximately 1200 to 700 cm-1 though sometimes extended to 1500-400 cm-1, contains dense pattern of single-bond stretches, in-plane and out-of-plane bending, and combination vibrations highly sensitive to molecular skeleton and substitution pattern. Even close structural isomers generate different fingerprints. Because absorption here is unique to overall molecular framework, comparison of this region with spectral libraries allows definitive identific

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.