Group decoding of mRNA occurs at
Group decoding or monitoring of codon-anticodon matches occurs at small subunit decoding center formed by 16S rRNA in prokaryotes and 18S rRNA in eukaryotes. Core residues A1492, A1493, G530 inspect shape of Watson-Crick pairs in minor groove via A-minor interactions, triggering GTPase activation only for cognate duplexes. Large subunit rRNAs 23S and 28S instead catalyze peptidyl transferase. Thus decoding fidelity segregates to small subunit rRNA, explaining aminoglycoside antibiotics inducing misreading by binding 16S decoding site and stabilizing near-cognate conformations. This refined reg
Ref: Watson Molecular Biology of the Gene, 7th ed., Chapter 15, Small subunit 16S rRNA decoding center function