Which of the following proteins allows nuclear translocation upon binding dexamethasone?
The glucocorticoid receptor is a cytosolic steroid receptor that undergoes ligand-dependent nuclear translocation. In the absence of hormone, it is sequestered in cytoplasm by Hsp90 chaperone complex. Binding of synthetic glucocorticoid dexamethasone induces conformational change, dissociation from chaperones, exposure of nuclear localization signal, and rapid translocation to nucleus to activate target genes. Fusing a protein of interest to the receptor's hormone-binding domain creates an inducible nuclear import system widely used to control transcription factor activity and study nucleocyto
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.