GITC is primarily used in RNA isolation for:
GITC (guanidinium isothiocyanate) is a powerful chaotropic agent used in RNA isolation methods like TRIzol. It simultaneously lyses cells, denatures proteins, and inactivates RNases instantly by disrupting hydrogen bonding and hydrophobic interactions, unfolding secondary structures. RNases are extremely resilient and require strong denaturants; GITC at 4 M denatures them irreversibly, protecting RNA from degradation. It does not primarily digest proteins enzymatically, inhibit DNases selectively, or solubilize lipids only. Combined with phenol, it allows partitioning of intact RNA into aqueou
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.