The band appearance with high quality and correct gel conditions is:
Band morphology reflects gel quality, electrical parameters, and sample integrity. Under optimal conditions, uniform pore distribution, proper buffer ionic strength, controlled voltage limiting Joule heating, and adequate polymerization produce minimal diffusion. DNA molecules of identical length migrate as tight zone, concentrating fluorescence in narrow region. This yields sharp, well-defined bands with high contrast. Smear indicates overloading, degradation, or excessive heating; faint suggests insufficient DNA; diffuse indicates poor polymerization or high electroendosmosis. Sharp appearan
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.