Equilibrium dialysis is used to study
Equilibrium dialysis separates free ligand from receptor-bound ligand by a semi-permeable membrane that retains the macromolecule. After equilibrium is established, measurement of ligand concentrations on both sides of the membrane allows calculation of bound and free concentrations. The method remains a classical, assumption-light approach for determining binding isotherms and extracting Kd and Bmax values.
Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)