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#enzyme-linked immunosorbent assay

3 public questions tagged with this topic.

Immunological Applications

ELISA is commonly used for:

Enzyme-linked immunosorbent assay revolutionized immunodiagnostics by converting antigen antibody binding into measurable enzymatic colorimetric signal without radioactivity, originally described by Eva Engvall and Peter Perlmann 1971. Polystyrene microtiter plate with high binding capacity via hydrophobic interaction adsorbs capture antibody coated overnight at alkaline pH 9.6 carbonate buffer 1 to 10 microgram per ml. Non-specific sites blocked with protein bovine serum albumin five percent, casein, or nonfat milk to prevent background adsorption through hydrophobic pockets. Sample containin

Ref: Engvall & Perlman Immunochemistry 1971 ELISA; Alberts MBoC ELISA HRP TMB sandwich principle diagnostic.

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Immunological Applications

ELISA is commonly used for:

ELISA pioneered Engvall Perlmann 1971 exploits highly specific high-affinity antigen-antibody interaction KD nanomolar picomolar detecting analytes complex biological fluids serum urine CSF culture supernatant. Principle immobilization one component passive hydrophobic adsorption polystyrene 96-well high-binding blocking nonspecific sites BSA casein preventing background. Indirect sandwich formats most sensitive plate coated capture antibody monoclonal high-affinity against target antigen human chorionic gonadotropin hCG pregnancy marker HIV p24 capsid antigen cytokines IL-6 TNF-alpha allergen

Ref: Engvall and Perlmann 1971 ELISA Original J Immunol; NCBI Bookshelf ELISA Principles; Janeway Immunoassay ELISA Technique Variants Chapter 3.

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