The binding site of a non-competitive inhibitor is:
An allosteric site is the accurate response regarding enzymatic activity or regulation described in this question. Enzymes are biological catalysts that accelerate reactions by lowering activation energy through specific substrate binding and transition state stabilization. In the context of Enzyme Inhibition, An allosteric site plays a specific catalytic or regulatory role determined by its active site configuration and mechanism of action. The other options (Always the active site, The substrate-binding pocket, and Between enzyme subunits) are either different enzymes with distinct substrate
Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 6