In situ bioremediation involves:
In situ bioremediation treats contamination directly within subsurface without excavation, preserving soil structure, minimizing disturbance to existing infrastructure and reducing expenses linked to transportation plus disposal. Treatment mobilizes indigenous microbes already adapted or introduced augmentative cultures capable of degrading target compounds within aquifer pores or vadose zone voids. Technologies include bioventing delivering low-flow air 1 to 3 cubic feet per minute per well to unsaturated zone to support aerobic oxidation of fuel hydrocarbons via alkane monooxygenase, biosparging injecting air below water table to increase dissolved oxygen up to 8 mg per L for dissolved-phase BTEX, and enhanced anaerobic bioremediation supplying lactate, molasses or emulsified vegetable oil as electron donors stimulating Dehalococcoides mediated reductive dechlorination of trichloroethene to ethene via tceA gene. Intrinsic processes monitored through natural attenuation also classify as in situ. Design requires detailed hydrogeologic characterization of hydraulic conductivity, groundwater velocity, redox zonation, and contaminant distribution to ensure uniform amendment delivery without channeling through high permeability preferential pathways. Although limited by heterogeneity causing inaccessible low permeability lenses, minimal footprint, lower worker exposure and ability to treat large volumes beneath buildings make in situ approach preferred when site conditions allow.
Ref: EPA In Situ Bioremediation Technical Guide 2013; Bouwer & Zehnder Soil bioremediation; PubMed in situ review.