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#dorsal gene

2 public questions tagged with this topic.

Mutant lacking dorsal gene becomes:

Dorsal protein, NF-kappa B homolog, provides ventralizing morphogen; its absence eliminates ventral and lateral fates causing entire embryo to adopt dorsal ectoderm identity characterized by amnioserosa and dorsal hairs circumferentially. Embryos lacking dorsal fail to form ventral furrow, mesoderm, or neuroectoderm, cuticle shows dorsalized phenotype with fine hairs everywhere, lethal before hatching. Ventralized phenotype arises from opposite genotype such as cactus loss or Toll gain. Anteriorized or posteriorized phenotypes involve bicoid or torso pathways. Dorsal loss therefore demonstrates necessity of NF-kappa B-like gradient for DV patterning, innate immunity cross-regulation, providing model linking developmental polarity and inflammatory signaling pathway conservation.

Ref: Gilbert, Developmental Biology, 12th ed., Chapter 9: dorsal null mutants become dorsalized - loss of ventral nuclear gradient.

The dorsal gene product in Drosophila primarily functions as:

Dorsal gene encodes transcription factor containing Rel homology domain homologous to mammalian NF-kB p50 and p65. Dorsal resides cytoplasmically bound to Cactus but translocates ventrally upon Toll activation, where concentration gradient specifies fates: high activates twist and snail for mesoderm, intermediate specifies neuroectoderm, low permits dorsal ectoderm. Its function encompasses DNA binding, activation and repression, not enzymatic catalysis or scaffolding. Receptor function belongs to Toll. Dorsal represents morphogen whose nuclear concentration interpreted through high and low affinity enhancers generates discrete DV thresholds during patterning.

Ref: Gilbert, Developmental Biology, 12th ed., Chapter 9: Dorsal as NF-kB transcription factor - DV morphogen gradient action.