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#DNA purification

2 public questions tagged with this topic.

ZOOLOGY

Which of the following is used to extract and purify DNA fragments from an agarose gel?

Elution is the process of extracting DNA fragments from an agarose gel for further use in recombinant DNA experiments. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Zoology section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Zoology portion (Latest NCERT Textbooks for Academic Session 2026-27 - Rationalized Edition for Class XI and XII), Topic: Structural organization, ph

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DNA & RNA isolation

Which method purifies DNA using magnetic particles?

Magnetic bead purification uses paramagnetic particles coated with silica or carboxyl groups that reversibly bind nucleic acids. Under high salt and chaotropic conditions, DNA adsorbs to bead surface via electrostatic and hydrophobic interactions. An external magnetic field immobilizes beads, allowing washing to remove proteins, salts, and contaminants. Elution in low salt buffer releases pure DNA. This method avoids centrifugation and toxic organics, is automatable, and yields high purity. It differs from column chromatography, CTAB extraction, and isopropanol precipitation which do not use m

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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