28 public questions tagged with this topic.
DNA polymerase is the enzyme responsible for synthesizing new DNA strands during replication. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.
Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.
DNA polymerase synthesizes new DNA in the 5' to 3' direction, not 3' to 5'. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.
Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.
Taq polymerase, derived from Thermus aquaticus, is a thermostable enzyme used in PCR to amplify DNA. This follows from NCERT principle where relation explains outcome clearly for students.
Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Biology - Zoology portion covering relevant system and function, Topic: Structural organization and physiology.
Taq polymerase is a thermostable DNA polymerase used in PCR to amplify DNA sequences.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Polymerase Chain Reaction - PCR and Applications
DNA polymerase is used to synthesize new DNA strands by adding nucleotides to a growing DNA chain, making it essential for DNA replication and PCR.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Polymerase Chain Reaction - PCR and Applications
DNA polymerase extends primers to synthesize new DNA strands during PCR.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Polymerase Chain Reaction - PCR and Applications
Helicase unwinds the DNA double helix, allowing replication and transcription to occur.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Tools - Restriction Enzymes - Molecular Scissors
DNA polymerase requires an RNA primer to initiate replication because it cannot start synthesis de novo.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance Post-Transcriptional Modifications - Capping Splicing Polyadenylation
DNA polymerase I removes RNA primers and fills the gaps with DNA nucleotides during replication.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance DNA Replication - Semiconservative and Machinery
DNA polymerase does not unwind the DNA helix; helicase is responsible for this function.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance DNA Replication - Semiconservative and Machinery
DNA polymerase III synthesizes the lagging strand in short Okazaki fragments during DNA replication.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance DNA Replication - Semiconservative and Machinery
DNA polymerase catalyzes the addition of nucleotides to the growing DNA strand during replication.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance DNA Replication - Semiconservative and Machinery
