Skip to content
Login Register

#DNA markers

2 public questions tagged with this topic.

Plant Breeding 4

SSR markers are:

Simple sequence repeats or microsatellites consist of tandem repeats of 1-6 bp motifs such as ATATAT or CAG repeats, abundant and evenly distributed across eukaryotic genomes. Variation in repeat number creates high polymorphism due to slippage during DNA replication by DNA polymerase. Primers flanking repeat amplify locus via PCR, and alleles differ in fragment length detectable by gel electrophoresis or capillary sequencing with fluorescent labels. Because both alleles at locus amplify and can be visualized simultaneously, SSR distinguishes homozygous from heterozygous state: single band for

Ref: Tautz D. Hypervariability of microsatellites; McCouch et al. 1997 Microsatellite markers in rice – co-dominant nature

View Details
Molecular Marker

Which one is not a molecular marker?

Molecular markers are defined as detectable DNA sequence variations that reveal polymorphism at the molecular level, including RFLP, RAPD, AFLP, SSR, SNP and similar techniques based directly on genome variation. Isozymes are multiple forms of enzymes differing in amino acid composition, charge and electrophoretic mobility, detected through biochemical staining after native gel electrophoresis. They reflect gene product variation rather than direct DNA polymorphism, influenced by post-translational modifications and tissue-specific expression. Though historically used, they are classified as b

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details