Skip to content
Login Register

#dilution

14 public questions tagged with this topic.

Microbial Physiology

A bacterial culture was diluted 1000-fold, and 0.1 mL of this sample was plated. If 100 colonies formed, what was the or

Viable count estimation via plate counting requires accounting for dilution magnitude and volume plated to infer original concentration. Only 30 to 300 colonies per plate statistically reliable. General formula colony forming units per mL equals colonies observed divided by product dilution factor fraction and volume plated in milliliters. If culture diluted 1000 fold meaning dilution factor 1 times ten to minus three, and 0.1 mL aliquot plated yielding 100 colonies, denominator equals ten to minus three times 0.1 equals ten to minus four. One hundred over ten to minus four equals one million

Ref: Prescott's Microbiology, 11th ed., Chapter 7: Viable count - CFU/mL = colonies/(dilution×volume).

View Details
Water and Mole Concept

How much water must be added to 10 mL of 0.5M HCl to dilute it tenfold?

90mL is obtained by applying the relevant formula or quantitative relationship to the given parameters. In Water and Mole Concept, numerical problem-solving requires understanding the mathematical relationships between biological variables. The calculation involves substituting the provided values into the appropriate equation and solving systematically. The other options (10mL, 100mL, and 990mL) result from common calculation errors such as using incorrect formulas, misidentifying variables, inverting ratios, or making arithmetic mistakes.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

View Details
Water and Mole Concept

If 500 mL of a 5M solution is diluted to 1500 mL, what is the final molarity?

1.5M accurately defines or describes the concept asked in this question. Within Water and Mole Concept, precise definitions and terminology are essential for clear scientific communication. The other options (1.66M, 0.017M, and 1.59M) either describe related but distinct concepts, use incorrect terminology, or confuse similar-sounding terms that have different scientific meanings. A thorough understanding of exact definitions helps distinguish between closely related biological concepts and is crucial for competitive examinations.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

View Details
pH and Buffer

What is the effect of diluting a buffer solution 10 times?

pH remains the same accurately defines or describes the concept asked in this question. Within pH and Buffer, precise definitions and terminology are essential for clear scientific communication. The other options (pH decreases, pH increases significantly, and Buffer is destroyed) either describe related but distinct concepts, use incorrect terminology, or confuse similar-sounding terms that have different scientific meanings. A thorough understanding of exact definitions helps distinguish between closely related biological concepts and is crucial for competitive examinations.

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

View Details