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#chromosomal analysis

2 public questions tagged with this topic.

Transcriptome analysis

M-FISH is most suitable for:

M-FISH generates a comprehensive spectral karyotype where each chromosome pair exhibits a distinct combinatorial fluorescent code. This permits detection of numerical aneuploidies, unbalanced translocations, marker chromosomes, and cryptic rearrangements that underlie inherited syndromes such as Down, Turner, Klinefelter, and microdeletion disorders. Because it interrogates entire genomes at chromosomal resolution rather than single nucleotide changes or protein interactions, it excels for constitutional cytogenetic screening. SNP identification requires sequencing or arrays, while transcript

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

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Transcriptome analysis

Which of these is used to analyze gross chromosomal rearrangements like translocations?

Multiplex FISH, or M-FISH, extends conventional FISH by using combinatorial labeling of whole-chromosome painting probes with distinct fluorochrome combinations, generating a unique spectral signature for each chromosome. This 24-color karyotyping allows simultaneous visualization of all chromosomes in different hues. Gross abnormalities such as reciprocal translocations, dicentric chromosomes, insertions, and complex rearrangements, which are difficult to resolve with single-locus probes or DNA microarrays and are not detectable by RAPD or RT-PCR, become readily identifiable as color junction

Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.

View Details